第一篇免疫學(xué)基礎(chǔ)實(shí)驗(yàn)/1實(shí)驗(yàn)一血清總補(bǔ)體溶血活性（CH50）的測(cè)定/1實(shí)驗(yàn)二人外周血單個(gè)核細(xì)胞的分離/3實(shí)驗(yàn)三E花環(huán)形成試驗(yàn)/5實(shí)驗(yàn)四中性粒細(xì)胞吞噬功能測(cè)定/7實(shí)驗(yàn)五雙向瓊脂擴(kuò)散試驗(yàn)/8實(shí)驗(yàn)六PCR�睸SP法檢測(cè)HLA�睞*02基因/9實(shí)驗(yàn)七利用SPA親和層析法分離純化IgG抗體/11實(shí)驗(yàn)八人血清總IgE檢測(cè)/12第二篇病原生物學(xué)基礎(chǔ)實(shí)驗(yàn)/14實(shí)驗(yàn)一細(xì)菌的生理/14實(shí)驗(yàn)二細(xì)菌分布及外界因素對(duì)細(xì)菌的影響/19實(shí)驗(yàn)三細(xì)菌形態(tài)結(jié)構(gòu)的檢查/22實(shí)驗(yàn)四化膿性球菌/24實(shí)驗(yàn)五腸道桿菌/26實(shí)驗(yàn)六厭氧性細(xì)菌/30實(shí)驗(yàn)七分枝桿菌/31實(shí)驗(yàn)八其他細(xì)菌/32實(shí)驗(yàn)九病毒/34實(shí)驗(yàn)十真菌/35實(shí)驗(yàn)十一內(nèi)毒素檢測(cè)（鱟試驗(yàn)）/37實(shí)驗(yàn)十二寄生關(guān)系及寄生蟲演化/40實(shí)驗(yàn)十三吸蟲/41實(shí)驗(yàn)十四絳蟲/44實(shí)驗(yàn)十五線蟲/46實(shí)驗(yàn)十六常見蠕蟲的檢驗(yàn)方法/49實(shí)驗(yàn)十七阿米巴/55實(shí)驗(yàn)十八孢子蟲/57實(shí)驗(yàn)十九鞭毛蟲/60實(shí)驗(yàn)二十纖毛蟲/62實(shí)驗(yàn)二十一原蟲檢查方法/62實(shí)驗(yàn)二十二醫(yī)學(xué)節(jié)肢動(dòng)物/65第三篇綜合性實(shí)驗(yàn)/71實(shí)驗(yàn)一多克隆抗體的制備及效價(jià)測(cè)定/71實(shí)驗(yàn)二流式細(xì)胞術(shù)檢測(cè)小鼠脾臟T、B細(xì)胞的比例/73實(shí)驗(yàn)三呼吸道感染常見病原微生物的實(shí)驗(yàn)室檢查/74實(shí)驗(yàn)四腸道桿菌的分離鑒定/78實(shí)驗(yàn)五泌尿生殖道感染常見病原微生物的實(shí)驗(yàn)室檢查/82實(shí)驗(yàn)六皮膚創(chuàng)傷感染常見細(xì)菌的實(shí)驗(yàn)室檢查/84實(shí)驗(yàn)七日本血吸蟲感染小鼠致病及免疫學(xué)診斷/86ContentsChapter 1Basic Experiments of Immunology/88Experiment 1Measure of the 50% Haemolytic Complement （CH50） Activity of Serum/88Experiment 2Isolation of Human Peripheral Blood Mononuclear Cells/91Experiment 3E�瞨osette Forming Test/92Experiment 4Neutrophil Phagocytosis Test/95Experiment 5Double Immunodiffusion Test/97Experiment 6Detect the HLA�睞*02 Gene by PCR�睸SP/98Experiment 7Purify IgG Antibody by SPA Affinity Chromatography/100Experiment 8Detection of Total IgE in Human Serum/102Chapter 2Basic Experiments of Pathogenic Biology/104Experiment 1Physiology of Bacteria/104Experiment 2The Distribution of Bacteria and the Effect of External Factors on Bacteria/112Experiment 3Examination of Bacterial Morphology and Structure/116Experiment 4Pyogenic Coccus/118Experiment 5Enterobacteriaceae/122Experiment 6Anaerobic bacteria/126Experiment 7Mycobacterium/127Experiment 8Other bacteria/129Experiment 9Virus/132Experiment 10Fungi/134Experiment 11Test of Endotoxin （Limulus Amoebocyte Lysate Test）/136Experiment 12Parasitic Relationships and Parasite Evolution/139Experiment 13Trematode/140Experiment 14Tapeworm/146Experiment 15Nematode/148Experiment 16Examination of Helminths/152Experiment 17Amoeba/160Experiment 18Sporozoa/163Experiment 19Flagellates/166Experiment 20Ciliates/168Experiment 21Methods for Protozoa Diagnosis/169Experiment 22Medical Arthropods/173Chapter 3Comprehensive Experiment/175Experiment 1Preparation and Titer Determination of Polyclonal Antibody/175Experiment 2Measure the Ratio of T and B Cells in the Spleen by Flow Cytometry/177Experiment 3Laboratory Examination of Common Pathogenic Microorganism of Respiratory Tract Infection/179Experiment 4Isolation and Identification of Enterobacteriaceae/184Experiment 5Laboratory Examination of Common Pathogens of Genitourinary Tract Infection/190Experiment 6Laboratory Examination of Common Bacteria in Skin Wound Infections/193Experiment 7Pathogenic and Immunological Diagnosis of Mice Infected with Schistosoma japonicum/196附錄A常用染色液和試劑的配制/199附錄B常用培養(yǎng)基的配制/201Appendix APreparation of Common Staining Solution and Reagents/203Appendix BPreparation of Common Culture Medium/205部分實(shí)驗(yàn)結(jié)果的彩圖/208Color Figures of Some Experimental Results/208參考文獻(xiàn)/225