Chapter1 Basic Knowledge of Analytical Chemistry Experiments1
1.1Tasks and Requirements of Analytical Chemistry Experiment1
1.2Laboratory Safety Knowledge2
1.3Basic Knowledge of Quantitative Analysis Experiments2
1.4Instruments and Basic Operations of Quantitative Analysis6
1.5Recording,Processing and Reporting of Experimental Data15

Chapter2 Sample Preparation and Separation Techniques20
2.1Preparation of Samples20
2.2Separation Techniques of Samples20

Chapter3 Basic Experiments for Quantitative Analysis29
Experiment 3.1Analytical Balance Weighing Exercise29
Experiment 3.2Basic Operation for Titration Analysis30
Experiment 3.3Calibration of Volumetric Instruments33
Experiment 3.4Preparation and Standardization of HCl and NaOH Solution35
Experiment 3.5Determination of Total Acidity in Edible Vinegar39
Experiment 3.6Determination of Nitrogen Content in Ammonium Salts—Formaldehyde Method41
Experiment 3.7Determination of Mixed Alkali—Double Indicator Method44
Experiment 3.8Preparation and Standardization of EDTA Solution47
Experiment 3.9Determination of Total Hardness of Tap Water50
Experiment 3.10Determination of Aluminum Content in Industrial Aluminum Sulfate53
Experiment 3.11Determination of Zn2+ and Bi3+ in Mixed Solution by Continuous Titration55
Experiment 3.12Preparation and Standardization of Potassium Permanganate Solution58
Experiment 3.13Determination of H2O2 Content in Hydrogen Peroxide Solution61
Experiment 3.14Determination of Iron in Iron Ore by Potassium Dichromate Method63
Experiment 3.15Preparation and Standardization of Iodine and Sodium Thiosulfate66
Experiment 3.16Determination of Glucose in Glucose Oral Solution72
Experiment 3.17Determination of Copper Content in Copper Alloys75
Experiment 3.18Determination of Chlorine in Tap Water by Mohr Method77
Experiment 3.19Determination of Nickel in Alloy Steel by Dimethylglyoxime Gravimetric Method79
Experiment 3.20Determination of Trace Iron with Phenanthroline by UV-Vis Spectroscopy82
Experiment 3.21Potentiometric Titration of Phosphoric Acid85

Chapter4 Comprehensive Design Experiments90
4.1Chemistry90
Experiment 4.1Determination of Benzoic Acid Content90
Experiment 4.2Determination of Phenol Content92
Experiment 4.3Determination of Purity of Soluble Barium Salt by Gravimetric Method95
Experiment 4.4Determination of Methanol Content in Industrial Alcohol98
Experiment 4.5Determination of Acetic Acid Content by Potentiometric Titration101
Experiment 4.6Determination of Iron，Aluminum，Calcium and Magnesium in Cement Clinker104
4.2Environment109
Experiment 4.7Determination of Total Alkalinity of Water109
Experiment 4.8Determination of Chemical Oxygen Demand（COD）in River Water111
Experiment 4.9Determination of Sulfate Content in Urban Sewage114
Experiment 4.10Determination of Formaldehyde in Waterborne Coatings by Spectrophotometry116
Experiment 4.11Determination of Glyphosate Content in Roundup120
4.3Biology121
Experiment 4.12Determination of Total Nitrogen Content for Compound Fertilizers121
Experiment 4.13Determination of Soil Organic Matter Content124
Experiment 4.14Determination of Chlorine Content for Compound Fertilizers127
Experiment 4.15Determination of Potassium Content for Compound Fertilizers 130
Experiment 4.16Determination of Available Phosphorus Content for Compound Fertilizers132
Experiment 4.17Determination of Available Phosphorus in Soil134
Experiment 4.18Macroporous Resin Adsorption and Spectrophotometric Determination of Flavonoids from Gingko Leaves137
Experiment 4.19Determination of Mixed Amino Acids Content 140
Experiment 4.20Determination of Reducing Sugar in Tobacco Leaf 142
4.4Pharmacy144
Experiment 4.21Determination of NH+4 in Chinese Medicine White Sal-Ammoniac 144
Experiment 4.22Determination of Calcium in Calcium Supplement by KMnO4 Method147
Experiment 4.23Determination of Al3+ and Mg2+ in Compound Aluminium Hydroxide Tablets150
Experiment 4.24Determination of the Content of Berberine Hydrochloride154
Experiment 4.25Determination of Acetaminophen in Ankahuangmin Capsules156
4.5Food158
Experiment 4.26Determination of Citric Acid Content in Commercially Available Citrus158
Experiment 4.27Determination of Calcium Content in Calcium Tablets/Milk Powder/Spinach161
Experiment 4.28Determination of NaCl Content in Soy Sauce163
Experiment 4.29Determination of Anthocyanin Content in Blueberries165
Experiment 4.30Determination of Melamine in Milk Products167
Experiment 4.31Determination of Vitamin B12 in Health Foods169

Chapter5 Innovation Experiments172
5.1Chemistry Experiment Competition for College Students172
Experiment 5.1Preparation of Rare Earth Europium（Eu） Complex and Determination of Coordination RatFio172
Experiment 5.2Preparation of Iron Oxide Nanoparticles and Its Application in the Determination of Melamine in Dairy Products175
Experiment 5.3Study on Synthesis of Complex\[Ni（Me3en）（acac）\]BPh4 and Its Solvent/Thermochromic Behavior177
Experiment 5.4Determination of Copper Content in Polynuclear Copper（Ⅰ） Complex180
5.2Research Training Experiments182
Experiment 5.5Gemini Ionic Liquid Modified Graphene Oxide Membranes for ReO-4/TcO-4Adsorption182
Experiment 5.6Colorimetric Determination of Uric Acid with Gold Nanoparticles183
Experiment 5.7A Green Chemistry Technique for Dissolution and Recovery of Gold from Electronic Wastes184
Experiment 5.8Extraction，Content Analysis and Cosmetic Application of Natural Pigment Betacyanin185

Appendix189
Appendix1Concentrations and Densities of Common Acid-Base Solutions189
Appendix2Common Primary Standard Substances and Their Drying Conditions and Applications189
Appendix3Preparation of Common Buffer190
Appendix4Common Indicators193
Appendix5Dissociation Constants of Weak Acids and Bases194
Appendix6Stability Constants of Coordination Compounds Formed by Metal Ions and EDTA（18-25℃，I=0.1 mol/L）198
Appendix7Standard Atomic Weights（1995，IUPAC）198


第1章分析化學(xué)實(shí)驗(yàn)基礎(chǔ)知識200
1.1分析化學(xué)實(shí)驗(yàn)的任務(wù)和要求200
1.2實(shí)驗(yàn)室安全知識200
1.3定量分析實(shí)驗(yàn)基礎(chǔ)知識201
1.4定量分析實(shí)驗(yàn)儀器及基本操作203
1.5實(shí)驗(yàn)數(shù)據(jù)的記錄、處理和實(shí)驗(yàn)報(bào)告210

第2章常用樣品制備及分離技術(shù)214
2.1樣品的制備214
2.2樣品的分離214

第3章定量分析基礎(chǔ)實(shí)驗(yàn)220
實(shí)驗(yàn)3.1分析天平的稱量練習(xí)220
實(shí)驗(yàn)3.2滴定分析的基本操作練習(xí)——酸堿滴定221
實(shí)驗(yàn)3.3容量儀器的校準(zhǔn)223
實(shí)驗(yàn)3.4酸堿溶液的配制與標(biāo)定225
實(shí)驗(yàn)3.5食用醋中總酸度的測定228
實(shí)驗(yàn)3.6銨鹽中氮含量的分析——甲醛法230
實(shí)驗(yàn)3.7混合堿的分析——雙指示劑法232
實(shí)驗(yàn)3.8EDTA溶液的配制與標(biāo)定234
實(shí)驗(yàn)3.9自來水硬度的測定236
實(shí)驗(yàn)3.10工業(yè)硫酸鋁中鋁含量的分析238
實(shí)驗(yàn)3.11鋅鉍混合溶液中Zn2+、Bi3+含量的連續(xù)測定240
實(shí)驗(yàn)3.12高錳酸鉀標(biāo)準(zhǔn)溶液的配制與標(biāo)定243
實(shí)驗(yàn)3.13雙氧水中過氧化氫含量的測定245
實(shí)驗(yàn)3.14鐵礦石中鐵含量的測定247
實(shí)驗(yàn)3.15碘和硫代硫酸鈉溶液的配制與標(biāo)定249
實(shí)驗(yàn)3.16葡萄糖口服液中葡萄糖含量的測定254
實(shí)驗(yàn)3.17銅合金中銅含量的測定256
實(shí)驗(yàn)3.18莫爾法測定自來水中的氯258
實(shí)驗(yàn)3.19丁二酮肟重量法測定合金鋼中的鎳260
實(shí)驗(yàn)3.20鄰二氮菲分光光度法測定微量鐵262
實(shí)驗(yàn)3.21磷酸的電位滴定265

第4章綜合設(shè)計(jì)性實(shí)驗(yàn)269
4.1化學(xué)類269
實(shí)驗(yàn)4.1苯甲酸含量的測定269
實(shí)驗(yàn)4.2苯酚含量的測定271
實(shí)驗(yàn)4.3重量法測定可溶性鋇鹽的純度273
實(shí)驗(yàn)4.4工業(yè)酒精中甲醇含量的測定275
實(shí)驗(yàn)4.5電位滴定法測定醋酸含量278
實(shí)驗(yàn)4.6水泥熟料中鐵、鋁、鈣、鎂含量的測定280
4.2環(huán)境類284
實(shí)驗(yàn)4.7水質(zhì)總堿度測定284
實(shí)驗(yàn)4.8河水中化學(xué)需氧量（COD）的測定286
實(shí)驗(yàn)4.9城市污水中硫酸鹽含量的測定288
實(shí)驗(yàn)4.10分光光度法測定水性涂料中的甲醛290
實(shí)驗(yàn)4.11農(nóng)達(dá)中草甘膦含量的測定293
4.3生物類294
實(shí)驗(yàn)4.12復(fù)合肥料中總氮含量的測定294
實(shí)驗(yàn)4.13土壤中有機(jī)質(zhì)含量的測定297
實(shí)驗(yàn)4.14復(fù)合肥料中氯離子含量的測定299
實(shí)驗(yàn)4.15復(fù)合肥料中鉀含量的測定301
實(shí)驗(yàn)4.16復(fù)合肥料中有效磷含量的測定302
實(shí)驗(yàn)4.17土壤中有效磷的測定304
實(shí)驗(yàn)4.18銀杏葉總黃酮含量的測定306
實(shí)驗(yàn)4.19混合氨基酸的測定309
實(shí)驗(yàn)4.20煙葉樣品中還原糖的測定310
4.4制藥類312
實(shí)驗(yàn)4.21中藥白硇砂中NH+4含量的測定312
實(shí)驗(yàn)4.22高錳酸鉀法測定補(bǔ)鈣劑中鈣含量314
實(shí)驗(yàn)4.23復(fù)方氫氧化鋁片中Al3+和Mg2+含量的測定317
實(shí)驗(yàn)4.24鹽酸黃連素含量的測定320
實(shí)驗(yàn)4.25氨咖黃敏膠囊中對乙酰氨基酚含量的測定321
4.5食品類323
實(shí)驗(yàn)4.26市售柑橘中檸檬酸含量的測定323
實(shí)驗(yàn)4.27鈣片/奶粉/菠菜中鈣含量的測定325
實(shí)驗(yàn)4.28醬油中NaCl含量的測定327
實(shí)驗(yàn)4.29藍(lán)莓中花青素含量的測定329
實(shí)驗(yàn)4.30奶制品中三聚氰胺的測定331
實(shí)驗(yàn)4.31保健食品中維生素B12的測定332

第5章探索創(chuàng)新實(shí)驗(yàn)335
5.1大學(xué)生化學(xué)競賽（分析化學(xué)部分）335
實(shí)驗(yàn)5.1稀土銪配合物的制備及配位比的測定335
實(shí)驗(yàn)5.2氧化鐵納米顆粒的制備及用于奶制品中三聚氰胺的測定337
實(shí)驗(yàn)5.3配合物\[Ni(Me3en)(acac)\]BPh4的合成及其溶劑/熱致變色行為研究339
實(shí)驗(yàn)5.4多核銅(Ⅰ)配合物中銅含量的測定341
5.2創(chuàng)新性實(shí)驗(yàn)342
實(shí)驗(yàn)5.5Gemini離子液體改性氧化石墨烯薄膜在ReO-4/TcO-4吸附中的應(yīng)用342
實(shí)驗(yàn)5.6金納米粒子比色法檢測尿酸343
實(shí)驗(yàn)5.7電子廢棄物中金元素的綠色溶解與提取344
實(shí)驗(yàn)5.8天然色素甜菜紅的提取、含量分析及其美妝應(yīng)用344

附錄349
附錄1常用酸堿的密度、含量和濃度349
附錄2常用基準(zhǔn)物質(zhì)的干燥條件和應(yīng)用349
附錄3常見緩沖溶液的配制350
附錄4常用酸堿指示劑及配制方法352
附錄5弱酸、弱堿的解離常數(shù)352
附錄6金屬離子與EDTA生成配位化合物的穩(wěn)定常數(shù)（18～25℃，I=0.1mol/L）356
附錄7原子表（1995，IUPAC）357

參考文獻(xiàn)358